2XE4

Structure of Oligopeptidase B from Leishmania major

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2008-06-10
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	95.478, 142.776, 208.919
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	117.851 - 1.650
R-factor	0.142
Rwork	0.140
R-free	0.17800
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.035
RMSD bond angle	2.353
Data reduction software	d*TREK
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.060	0.460
Number of reflections	167772
<I/σ(I)>	8.8	1.7
Completeness [%]	98.4	100
Redundancy	3.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.2		PROTEIN WAS DIALYZED INTO 50 MM TRIS, PH 8.0 AND INCUBATED WITH 10 MM ANTIPAIN FOR 30 MINS. THIS WAS MIXED IN A 1:1 RATIO WITH 25% 1,2 PROPANEDIOL, 10% GLYCEROL, 5 % PEG300 AND 0.1 M PHOSPHATE CITRATE, PH 4.2
1		4.2		PROTEIN WAS DIALYZED INTO 50 MM TRIS, PH 8.0 AND INCUBATED WITH 10 MM ANTIPAIN FOR 30 MINS. THIS WAS MIXED IN A 1:1 RATIO WITH 25% 1,2 PROPANEDIOL, 10% GLYCEROL, 5 % PEG300 AND 0.1 M PHOSPHATE CITRATE, PH 4.2