2X7K
The crystal structure of PPIL1 in complex with cyclosporine A suggests a binding mode for SKIP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-21 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 103.218, 35.701, 45.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.277 - 1.150 |
R-factor | 0.1292 |
Rwork | 0.128 |
R-free | 0.14870 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 2.205 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.250 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.086 | 0.440 |
Number of reflections | 62933 | |
<I/σ(I)> | 8.9 | 2 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 6.7 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 0.1 M HEPES, PH 7.2, 0.7 M SODIUM ACETATE, 15 MM CDCL2 AND 50 MM GUANIDINE-HCL GROWN AT 4DEGC |