2X7D
Crystal structure of human kinesin Eg5 in complex with (S)-dimethylenastron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.515, 79.717, 159.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.580 - 2.300 |
| R-factor | 0.17921 |
| Rwork | 0.176 |
| R-free | 0.24053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x88 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.649 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.390 |
| Number of reflections | 40030 | |
| <I/σ(I)> | 16 | 5.5 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 7.9 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30% PEG 3350, 0.2M K2HPO4, 0.1M HEPES PH 8.0 |
