2X63
Crystal structure of the sialyltransferase CST-II N51A in complex with CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-18 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 116.190, 116.190, 46.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.000 |
| R-factor | 0.18788 |
| Rwork | 0.186 |
| R-free | 0.23005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ro8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.986 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 21401 | |
| <I/σ(I)> | 16.6 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES5, PH 7.5, 8% (W/V) POLYETHYLENE GLYCOL 6000, 5% (V/V) 2-METHYL-2,4-PENTANEDIOL. |
