2X44
Structure of a strand-swapped dimeric form of CTLA-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-17 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 42.951, 42.951, 140.101 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.600 - 2.600 |
| R-factor | 0.19385 |
| Rwork | 0.192 |
| R-free | 0.24501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i81 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.040 | 0.200 |
| Number of reflections | 4289 | |
| <I/σ(I)> | 29.1 | 9 |
| Completeness [%] | 85.0 | 45.8 |
| Redundancy | 22.1 | 21.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.02 M MAGNESIUM CHLORIDE, 0.1 M HEPES PH 7.5, 22 % W/V POLYACRYLIC ACID 5100 SODIUM SALT, 0.4 M NDSB-256 (OR 6 % 1,6-DIAMINOHEXANE). |
