2X2U
First two Cadherin-like domains from Human RET
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-04 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.946, 1.514 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 86.074, 86.074, 110.475 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.860 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.22800 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.587 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.860 |
| Number of reflections | 33291 | |
| <I/σ(I)> | 20.7 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.6 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 288 | 1.6 M (NH4)2SO4, 50 MM TRIS PH 7.5, 4% (V/V) 1\,4 BUTANEDIOL AT 15 DEG C. |
