2WVR
Human Cdt1:Geminin complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 92.804, 92.804, 164.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.413 - 3.300 |
| R-factor | 0.2433 |
| Rwork | 0.240 |
| R-free | 0.30070 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.790 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.060 | 0.650 |
| Number of reflections | 11298 | |
| <I/σ(I)> | 21.9 | 3 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PH 7.5 100-150 MM NACL CAPILLARY GRADIENT |
