2WV4
Crystal structure of foot-and-mouth disease virus 3C protease in complex with a decameric peptide corresponding to the VP1-2A cleavage junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.760, 75.250, 85.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.740 - 2.500 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j92 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.300 | 2.660 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.140 | 0.350 |
Number of reflections | 14044 | |
<I/σ(I)> | 9.1 | 2.5 |
Completeness [%] | 93.4 | 78.5 |
Redundancy | 4.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | SEE PAPER, pH 7 |