2WTF
DNA polymerase eta in complex with the cis-diammineplatinum (II) 1,3- GTG intrastrand cross-link
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 103.240, 103.240, 292.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.500 |
| R-factor | 0.22922 |
| Rwork | 0.227 |
| R-free | 0.26871 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jih |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.633 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 55019 | |
| <I/σ(I)> | 25.1 | 5.8 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 8.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4MG/ML, 11-14% PEG3350, 0.15-0.2M CACL2, 0.1M ATP |
