2WO6
Human Dual-Specificity Tyrosine-Phosphorylation-Regulated Kinase 1A in complex with a consensus substrate peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-15 |
Detector | ADSC CCD |
Spacegroup name | P 65 |
Unit cell lengths | 168.430, 168.430, 62.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.500 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vx3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.528 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.130 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.170 | 0.520 |
Number of reflections | 35283 | |
<I/σ(I)> | 10.8 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2M SODIUM FORMATE, 20% PEG3350, 10% ETHYLENE GLYCOL, pH 7.5 |