2WO2
Crystal Structure of the EphA4-ephrinB2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-20 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 61 |
| Unit cell lengths | 107.371, 107.371, 47.619 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 2.450 |
| R-factor | 0.21784 |
| Rwork | 0.216 |
| R-free | 0.26021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kgy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.050 | 0.530 |
| Number of reflections | 11645 | |
| <I/σ(I)> | 43.2 | 2.6 |
| Completeness [%] | 99.3 | 94.2 |
| Redundancy | 11.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 30% PEG 6000, 1 M LITHIUM CHLORIDE, AND 100 MM MES PH 6.0 |
