2WNS
Human Orotate phosphoribosyltransferase (OPRTase) domain of Uridine 5' -monophosphate synthase (UMPS) in complex with its substrate orotidine 5'-monophosphate (OMP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.400, 43.600, 71.000 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 37.060 - 1.900 |
| R-factor | 0.18244 |
| Rwork | 0.180 |
| R-free | 0.22791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zyk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.437 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.060 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.570 |
| Number of reflections | 28358 | |
| <I/σ(I)> | 14.8 | 3.6 |
| Completeness [%] | 98.4 | 98.3 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
