2WIN

C3 convertase (C3bBb) stabilized by SCIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-02
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	228.632, 121.491, 280.783
Unit cell angles	90.00, 91.64, 90.00

Refinement procedure

Resolution	39.675 - 3.900
R-factor	0.2533
Rwork	0.253
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2A74 2i07 1rrk 2qff
RMSD bond length	0.002
RMSD bond angle	0.519
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	4.100
High resolution limit [Å]	3.900	3.900
Rmerge	0.130	0.690
Number of reflections	137476
<I/σ(I)>	10.4	1.8
Completeness [%]	97.6	92
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		75 MM SODIUM/POTASSIUM TARTRATE, 8.0% PEG 3350, 50 MM BIS-TRIS PROPANE, PH 6.5