2WGX
HUMAN P53 CORE DOMAIN MUTANT M133L-V203A-Y236F-N239Y-T253I-N268D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.328, 71.075, 104.915 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.607 - 1.750 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uol |
RMSD bond length | 0.008 |
RMSD bond angle | 1.178 |
Phasing software | CNS |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.400 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.080 | 0.280 |
Number of reflections | 48639 | |
<I/σ(I)> | 17.7 | 5.5 |
Completeness [%] | 97.6 | 92.4 |
Redundancy | 6.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 294 | SITTING DROP VAPOR DIFFUSION AT 21 DEGREE C. PROTEIN SOLUTION: 6 MG/ML IN 25 MM SODIUM PHOSPHATE PH 7.2, 150 MM KCL, 5 MM DTT. RESERVOIR BUFFER: 100 MM HEPES PH 7.2, 19 % PEG 4000, 5 MM DTT. |