2WG3
Crystal structure of the complex between human hedgehog-interacting protein HIP and desert hedgehog without calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.821, 110.701, 144.381 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.227 |
Rwork | 0.224 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2WFQ AND 2WFT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.180 | 0.810 |
Number of reflections | 49775 | |
<I/σ(I)> | 15.3 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.4 M SODIUM/POTASSIUM PHOSPHATE, PH 5.0 |