2WDT
Crystal structure of Plasmodium falciparum UCHL3 in complex with the suicide inhibitor UbVME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 134.400, 75.300, 85.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.300 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xd3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.319 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.450 |
| Number of reflections | 39592 | |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 98.1 | 90.2 |
| Redundancy | 5.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 100 MM BIS-TRIS PH 5.5, 200 MM NACL, 25 % PEG 3350 |
