2WD0
CRYSTAL STRUCTURE OF NONSYNDROMIC DEAFNESS (DFNB12) ASSOCIATED MUTANT D124G OF MOUSE CADHERIN-23 EC1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 179.693, 179.693, 63.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.770 - 2.740 |
| R-factor | 0.22089 |
| Rwork | 0.219 |
| R-free | 0.24865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wcp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 28267 | |
| <I/σ(I)> | 13.8 | 4.47 |
| Completeness [%] | 97.9 | 97.4 |
| Redundancy | 7.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 0.1M SODIUM CACODYLATE PH 7.1, 1M NACL |
