2WCO

Structures of the Streptomyces coelicolor A3(2) Hyaluronan Lyase in Complex with Oligosaccharide Substrates and an Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	140.110, 140.110, 100.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 1.940
R-factor	0.17104
Rwork	0.170
R-free	0.19284
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	1.896
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0082)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.940
Rmerge	0.080
Number of reflections	63596
Completeness [%]	89.4
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				4M NA-FORMATE