2WBS
Crystal structure of the zinc finger domain of Klf4 bound to its target DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91841, 2.0, 1.28204, 1.28312, 1.16967 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.000, 45.970, 73.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.644 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX (SHARP REFMAC5 COOT) |
| Refinement software | REFMAC (5.5.0082) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 15421 | |
| <I/σ(I)> | 12.2 | 2.7 |
| Completeness [%] | 97.5 | 98.6 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
