2W2X

Complex of Rac2 and PLCg2 spPH Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-12
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	131.570, 84.459, 74.196
Unit cell angles	90.00, 112.21, 90.00

Refinement procedure

Resolution	69.406 - 2.300
R-factor	0.237
Rwork	0.234
R-free	0.29440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UPQ AND 2OVJ
RMSD bond length	0.010
RMSD bond angle	1.406
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.500
Number of reflections	29046
<I/σ(I)>	12.8	1.9
Completeness [%]	86.9	52.7
Redundancy	3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	277	RAC2(2-177)GTPGS-PLCSPPH(Y495F) COMPLEX WAS CRYSTALLIZED USING A PROTEIN CONCENTRATION OF 25 MG/ML WITH PRECIPITANT (18% PEG1500, 10% GLYCEROL, 100MM SPG PH9) BY MICRO-SEEDING AT A CONSTANT TEMPERATURE OF 4C