2W2V
Rac2 (G12V) in complex with GTPgS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-13 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.202, 94.529, 107.237 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 53.620 - 2.000 |
R-factor | 0.283 |
Rwork | 0.280 |
R-free | 0.34500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN HOUSE RAC2 STRUCTURE |
RMSD bond length | 0.002 |
RMSD bond angle | 0.641 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.000 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.080 | 0.520 |
Number of reflections | 79004 | |
<I/σ(I)> | 12.9 | 2.3 |
Completeness [%] | 98.7 | 96.1 |
Redundancy | 4.8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | RAC2(2-177)GTPGS WAS CRYSTALLIZED USING A PROTEIN CONCENTRATION OF 25 MG/ML WITH PRECIPITANT (18% PEG3350, 300 MM SODIUM/POTASSIUM TARTRATE) AND AT A CONSTANT TEMPERATURE OF 20C |