2VXN
E65Q-TIM complexed with phosphoglycolohydroxamate at 0.82 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.360, 50.650, 58.660 |
| Unit cell angles | 90.00, 117.56, 90.00 |
Refinement procedure
| Resolution | 10.000 - 0.820 |
| R-factor | 0.092 |
| R-free | 0.10300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n55 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 0.031 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 0.830 |
| High resolution limit [Å] | 0.820 | 0.820 |
| Rmerge | 0.050 | 0.270 |
| Number of reflections | 223330 | |
| <I/σ(I)> | 22.96 | 6.12 |
| Completeness [%] | 89.6 | 84.8 |
| Redundancy | 5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 24.1 % PEG6000, 0.1 M ACETIC ACID PH 4.5, 1 MM DTT, 1 MM EDTA, 1 MM NAN3 |
