2VUZ
Crystal structure of Codakine in complex with biantennary nonasaccharide at 1.7A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 32.163, 100.197, 95.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.130 - 1.700 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vuv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.763 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.600 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 17204 | |
| <I/σ(I)> | 5.3 | 2 |
| Completeness [%] | 98.6 | 99.3 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M LITHIUM SULFATE IN 100 MM SODIUM ACETATE PH 7.5 WITH 15% PEG 4000 |
