2VR3
Structural and Biochemical Characterization of Fibrinogen binding to ClfA from Staphylocccus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.427, 61.836, 81.777 |
| Unit cell angles | 85.44, 81.84, 82.45 |
Refinement procedure
| Resolution | 15.000 - 1.950 |
| R-factor | 0.21264 |
| Rwork | 0.211 |
| R-free | 0.27931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n67 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.642 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.198 |
| Number of reflections | 49456 | |
| <I/σ(I)> | 5.8 | 2.7 |
| Completeness [%] | 93.9 | 88.7 |
| Redundancy | 1.9 | 1.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 16-20% PEG 8000, 100MM SUCCINIC ACID PH 6.0 |
