2VQY
Structure of AAC(6')-Ib in complex with Parmomycin and AcetylCoA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-06 |
| Detector | RIGAKU-MSC RAXIS-IV |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.331, 57.331, 147.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 147.440 - 1.800 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21700 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.537 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.670 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.220 |
| Number of reflections | 23603 | |
| <I/σ(I)> | 34.6 | 5.4 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.75 | 291 | PROTEIN (10 MG/ML, 20 MM TRIS PH 7.5, 20 MM NACL, 3 MM ACCOA) PRECIPITANT (20% PEG3350, 200 MM CAACETATE, 100 MM NACACODYLATE PH 6.75) SOAK 10 MM PARMOMYCIN GROWN BY VAPOR DIFFUSION UNDER OIL AT 18 DEGREES CELSIUS. |
