2VQW
Structure of inhibitor-free HDAC4 catalytic domain (with gain-of- function mutation His332Tyr)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 137.911, 137.911, 69.519 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.033 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.480 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.150 | 0.840 |
| Number of reflections | 15460 | |
| <I/σ(I)> | 14.3 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 18% PEG 10000, 1MM DTT |
