2VPP
Drosophila melanogaster deoxyribonucleoside kinase successfully activates gemcitabine in transduced cancer cell lines
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-08 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 120.106, 62.298, 68.078 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j90 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.377 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.260 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.340 |
Number of reflections | 24968 | |
<I/σ(I)> | 12 | 3.8 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |