2VKZ
Structure of the cerulenin-inhibited fungal fatty acid synthase type I multienzyme complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 231.900, 231.900, 756.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.993 - 4.000 |
R-factor | 0.2679 |
Rwork | 0.268 |
R-free | 0.26750 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 4.220 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.240 | 1.000 |
Number of reflections | 169821 | |
<I/σ(I)> | 7 | 1.4 |
Completeness [%] | 98.0 | 98 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |