2VHS
Cathsilicatein, a chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.826, 58.102, 70.209 |
| Unit cell angles | 105.67, 104.97, 105.05 |
Refinement procedure
| Resolution | 63.250 - 1.500 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mhw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.981 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.290 |
| Number of reflections | 108723 | |
| <I/σ(I)> | 14.2 | 2.1 |
| Completeness [%] | 92.0 | 74 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6 |
