2VFG
Crystal structure of the F96H mutant of Plasmodium falciparum triosephosphate isomerase with 3-phosphoglycerate bound at the dimer interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-08-14 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.197, 47.136, 109.167 |
Unit cell angles | 90.00, 96.65, 90.00 |
Refinement procedure
Resolution | 23.780 - 1.950 |
R-factor | 0.21028 |
Rwork | 0.208 |
R-free | 0.24819 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o5x |
RMSD bond length | 0.006 |
RMSD bond angle | 0.874 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.100 | 0.440 |
Number of reflections | 62256 | |
<I/σ(I)> | 9.8 | 1.97 |
Completeness [%] | 93.3 | 82.3 |
Redundancy | 2.7 | 2.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 0.1 M SODIUM ACETATE PH 4.0-5.5, PEG 1450 8-24% |