2V9Y
Human aminoimidazole ribonucleotide synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.670, 80.990, 98.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.100 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cli |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.477 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 38171 | |
| <I/σ(I)> | 28.7 | 8.06 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 14.8 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 0.1 M BIS-TRIS PH 5.2, 27% PEG 3350, 0.2 M AMMONIUM SULFATE, 0.2 MG/ML OF CHYMOTRYPSIN WAS ADDED PRIOR TO CRYSTALLISATION. |
