2UZR
A transforming mutation in the pleckstrin homology domain of AKT1 in cancer (AKT1-PH_E17K)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-17 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.886, 32.811, 42.160 |
Unit cell angles | 90.00, 119.11, 90.00 |
Refinement procedure
Resolution | 36.640 - 1.940 |
R-factor | 0.239 |
Rwork | 0.238 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1unq |
RMSD bond length | 0.005 |
RMSD bond angle | 0.877 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.640 | 2.020 |
High resolution limit [Å] | 1.940 | 1.950 |
Rmerge | 0.060 | 0.139 |
Number of reflections | 7529 | |
<I/σ(I)> | 4.9 | 2.11 |
Completeness [%] | 97.1 | 86.4 |
Redundancy | 4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 0.1 M HEPES PH 7.5 AND 1.4 M SODIUM CITRATE, OR 0.1 M SODIUM ACETATE PH 4.6, 0.2 M AMMONIUM ACETATE AND 15%-30% PEG 3350, |