2UZJ
Crystal structure of the mature streptococcal cysteine protease, mSpeB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-15 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.828, 60.888, 70.365 |
Unit cell angles | 90.00, 101.14, 90.00 |
Refinement procedure
Resolution | 21.480 - 1.550 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dki |
RMSD bond length | 0.015 |
RMSD bond angle | 1.341 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | CCP4I |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.480 | 1.590 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.090 | 0.770 |
Number of reflections | 63795 | |
<I/σ(I)> | 15.32 | 0.97 |
Completeness [%] | 90.1 | 55.9 |
Redundancy | 4.7 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M (NH4)2SO4, 0.1 M NA-ACETATE, PH 4.0, 20 % PEG4000. PROTEIN KONCENTRATION WAS 0.07 MM (IN 20 MM NA-ACETATE, PH 5.0). |