2UZJ

Crystal structure of the mature streptococcal cysteine protease, mSpeB

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006508	biological_process	proteolysis
A	0008234	molecular_function	cysteine-type peptidase activity
B	0006508	biological_process	proteolysis
B	0008234	molecular_function	cysteine-type peptidase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE E64 A 1254

Chain	Residue
A	GLN17
A	HOH2166
B	GLN36
B	SER37
A	CYS47
A	ASP130
A	GLY136
A	SER137
A	GLY188
A	GLY194
A	HIS195
A	HOH2165

---

site_id	AC2
Number of Residues	19
Details	BINDING SITE FOR RESIDUE E64 B 1254

Chain	Residue
A	GLN36
A	SER37
B	GLN17
B	CYS47
B	VAL48
B	ASP128
B	ASP130
B	SER135
B	GLY136
B	SER137
B	ALA138
B	GLN187
B	GLY194
B	HIS195
B	HOH2018
B	HOH2092
B	HOH2099
B	HOH2166
B	HOH2167

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:19712682, ECO:0000269|PubMed:22645124, ECO:0000305|PubMed:8675287

Chain	Residue	Details
A	CYS47
B	CYS47

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000269|PubMed:19712682

Chain	Residue	Details
A	HIS195
B	HIS195

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:22645124, ECO:0007744|PDB:4D8E

Chain	Residue	Details
A	SER137
B	SER137
A	GLN17
A	HIS195
B	GLN17
B	HIS195

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000305|PubMed:22645124, ECO:0007744|PDB:4D8I

Chain	Residue	Details
A	GLY194
B	GLY194

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Cysteine methyl disulfide; in zymogen form => ECO:0000269|PubMed:6381494

Chain	Residue	Details
A	CYS47
B	CYS47

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: covalent => ECO:0000269|PubMed:19712682, ECO:0000269|PubMed:22645124, ECO:0007744|PDB:2UZJ, ECO:0007744|PDB:4D8E

Chain	Residue	Details
A	CYS47
B	CYS47

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1dki

Chain	Residue	Details
A	CYS47
A	HIS195

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1dki

Chain	Residue	Details
B	CYS47
B	HIS195

---

site_id	MCSA1
Number of Residues	3
Details	M-CSA 598

Chain	Residue	Details
A	CYS47
A	HIS195	proton shuttle (general acid/base)
A	TRP212

---

site_id	MCSA2
Number of Residues	3
Details	M-CSA 598

Chain	Residue	Details
B	CYS47
B	HIS195	proton shuttle (general acid/base)
B	TRP212

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