2SSP
LEUCINE-272-ALANINE URACIL-DNA GLYCOSYLASE BOUND TO ABASIC SITE-CONTAINING DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Collection date | 1996-08-23 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 120.300, 48.800, 65.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1akz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.082 * | 0.231 * |
| Total number of observations | 66017 * | |
| Number of reflections | 18564 | |
| <I/σ(I)> | 15.6 | 4.7 |
| Completeness [%] | 97.8 | 94.2 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 20% PEG 4000, 100 MM HEPES PH 6.5, 10% DIOXANE, 1 MM DITHIOTHREITOL, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | DIOXANE | ||
| 4 | 1 | 1 | DITHIOTHREITOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | 100 (mM) | pH6.5 | |
| 3 | 1 | reservoir | dioxane | 10 (%) | |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) |
