2RGT
Crystal Structure of Lhx3 LIM domains 1 and 2 with the binding domain of Isl1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.494, 62.218, 51.885 |
| Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.050 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.25400 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.919 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.760 | 40.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 4.410 | 2.050 |
| Rmerge | 0.051 | 0.024 | 0.388 |
| Number of reflections | 23120 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 96.4 | 99.4 | 78.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapour diffusion, Hanging drop | 7.25 | 298 | 1.0M tri-sodium citrate, 0.1M Tris, HCl pH 7.25, Vapour diffusion, Hanging drop, temperature 298K |
