2RFD

Crystal structure of the complex between the EGFR kinase domain and a Mig6 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-06
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	143.964, 143.964, 237.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.010 - 3.600
Rwork	0.234
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gs7
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.730
High resolution limit [Å]	3.600	7.750	3.600
Rmerge	0.136	0.035	0.454
Number of reflections	17156
<I/σ(I)>	15.7
Completeness [%]	98.1	97.7	88.1
Redundancy	7.6	7.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG3350, 200 mM Na2SO4, 100 mM Bis-Tris propane, pH 7.5, vapor diffusion, hanging drop, temperature 293K