2RD7

Human Complement Membrane Attack Proteins Share a Common Fold with Bacterial Cytolysins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9565
Spacegroup name	P 21 21 2
Unit cell lengths	96.553, 126.087, 51.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.000 - 2.150
R-factor	0.20827
Rwork	0.206
R-free	0.26086
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Lower resolution model solved by SAD
RMSD bond length	0.018
RMSD bond angle	1.630
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.150	2.150
Rmerge	0.048	0.400
Number of reflections	32462
<I/σ(I)>	33.4	2.6
Completeness [%]	93.2	84.2
Redundancy	4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	6% PEG 20,000, 0.1 M citric acid, 0.025 M Mg(NO3)2, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K