2RD0
Structure of a human p110alpha/p85alpha complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.081 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.058, 117.050, 151.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.370 - 3.050 |
| R-factor | 0.267 |
| Rwork | 0.263 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.483 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 38994 | |
| <I/σ(I)> | 9.4 | 2.1 |
| Completeness [%] | 98.7 | 88.2 |
| Redundancy | 7.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | Sodium Formate, pH 7.0, VAPOR DIFFUSION, temperature 293K |
