2RB3
Crystal Structure of Human Saposin D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.154, 41.088, 65.929 |
| Unit cell angles | 89.96, 80.84, 71.49 |
Refinement procedure
| Resolution | 21.670 - 2.100 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27400 |
| Starting model (for MR) | 2r0r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.247 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.051 | 0.028 | 0.185 |
| Number of reflections | 21734 | ||
| <I/σ(I)> | 22 | ||
| Completeness [%] | 94.5 | 97.6 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 291 | 100 mM BisTris, 2.3 M ammonium sulfate, 100 mM urea, pH 5.9, vapor diffusion, hanging drop, temperature 291K |
