2R7G
Structure of the retinoblastoma protein pocket domain in complex with adenovirus E1A CR1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Detector technology | CCD |
| Collection date | 2006-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.713, 57.706, 92.268 |
| Unit cell angles | 94.34, 92.69, 105.79 |
Refinement procedure
| Resolution | 40.820 - 1.671 |
| R-factor | 0.19759 |
| Rwork | 0.195 |
| R-free | 0.21823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.256 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.671 | 3.600 | 1.671 |
| Rmerge | 0.035 | 0.021 | 0.392 |
| Number of reflections | 121240 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 92.0 | 98.4 | 56.7 |
| Redundancy | 2.5 | 2.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 15mM magnesium acetate tetrahydrate, 50mM sodium cacodylate trihydrate, pH 6.0 and 1.7M ammonium sulfate, VAPOR DIFFUSION, temperature 293K |
