2R3A
Methyltransferase domain of human suppressor of variegation 3-9 homolog 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.748, 63.925, 64.529 |
| Unit cell angles | 90.00, 109.33, 90.00 |
Refinement procedure
| Resolution | 35.380 - 2.000 |
| R-factor | 0.17851 |
| Rwork | 0.177 |
| R-free | 0.21252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1MHV |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.551 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 22956 | |
| <I/σ(I)> | 15 | 3.4 |
| Completeness [%] | 96.9 | 92.9 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 10000, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
