2R27
Constitutively zinc-deficient mutant of human superoxide dismutase (SOD), C6A, H80S, H83S, C111S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-01-15 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.08 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.200, 35.900, 68.300 |
Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
Resolution | 19.200 - 2.000 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n18 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.200 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.364 | |
Number of reflections | 15899 | |
Completeness [%] | 91.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2.45 M ammonium sulfate, 200 mM NaCl in 50 mM Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |