2R1H
met-Trout IV hemoglobin at pH 6.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.063, 62.998, 77.016 |
| Unit cell angles | 90.00, 92.20, 90.00 |
Refinement procedure
| Resolution | 76.920 - 1.900 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.22000 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.408 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 76.900 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 42904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | 20% PEG 4K, 0.1 M BisTris , pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
