2QNA
Crystal structure of human Importin-beta (127-876) in complex with the IBB-domain of Snurportin1 (1-65)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.220, 79.070, 208.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.840 |
| R-factor | 0.23526 |
| Rwork | 0.233 |
| R-free | 0.28247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.881 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3104.260 | 2.920 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.192 | 0.626 |
| Number of reflections | 25868 | |
| <I/σ(I)> | 9.18 | 2.55 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.82 | 4.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.55 | 283 | sodium citrate, Ammonium SO4, Ethanol, NaCl, Tris, pH 5.55, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
