2QL1

Structural Characterization of a Mutated, ADCC-Enhanced Human Fc Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	2007-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	49.869, 147.492, 74.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.250 - 2.534
R-factor	0.23506
Rwork	0.232
R-free	0.29287
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2DTQ
RMSD bond length	0.016
RMSD bond angle	1.880
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.250	2.590
High resolution limit [Å]	2.530	2.530
Rmerge	0.159	0.614
Number of reflections	8675
<I/σ(I)>	7.5	1.9
Completeness [%]	92.6	100
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	8% PEG 3350, 200 mM Zn Acetate, 5% Glycerol, 0.1 M Imidazole -Malate buffer, pH 8.0, VAPOR DIFFUSION, temperature 298.0K