2QKI
Human C3c in complex with the inhibitor compstatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07230 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.809, 124.754, 127.367 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.400 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.28093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a74 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.408 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.067 | 0.435 |
| Number of reflections | 100641 | |
| <I/σ(I)> | 13.4 | 2.4 |
| Completeness [%] | 96.4 | 98 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG-MME 2000, KBr, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
