2QK4
Human glycinamide ribonucleotide synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.280, 79.810, 113.870 |
| Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.450 |
| R-factor | 0.17386 |
| Rwork | 0.171 |
| R-free | 0.22107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gso |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.546 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.049 | 0.341 |
| Number of reflections | 44786 | |
| <I/σ(I)> | 22.15 | 3.94 |
| Completeness [%] | 99.2 | 91.7 |
| Redundancy | 5.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 16% PEG 3350, 0.3M LiSO4, 100 mM Bis-Tris pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
