2QHO
Crystal structure of the UBA domain from EDD ubiquitin ligase in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08090 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.849, 59.333, 246.672 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.530 - 1.850 |
R-factor | 0.20922 |
Rwork | 0.207 |
R-free | 0.25807 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq 2ooa |
RMSD bond length | 0.018 |
RMSD bond angle | 1.720 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.051 | 0.292 |
Number of reflections | 38635 | |
<I/σ(I)> | 29.5 | 7.1 |
Completeness [%] | 92.9 | 81 |
Redundancy | 7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M citric acid, 20% PEG 6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |