2QFX
Crystal structure of Saccharomyces cerevesiae mitochondrial NADP(+)-dependent isocitrate dehydrogenase in complex with NADPH, a-ketoglutarate and Ca(2+)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-11-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 113.415, 99.630, 129.950 |
| Unit cell angles | 90.00, 111.21, 90.00 |
Refinement procedure
| Resolution | 49.730 - 2.700 |
| Rwork | 0.217 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qfw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.049 | 0.540 |
| Number of reflections | 73998 | |
| <I/σ(I)> | 15 | 3.2 |
| Completeness [%] | 98.6 | 97.6 |
| Redundancy | 5.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 100mM Tris-HCl, 25% PEG 4000, 0.2M Na-acetate, 4% 1,3-propanediol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
